biotinylated anti-rat igd  (Bio-Rad)

Journal: iScience

Article Title: High affinity mAb infusion can enhance maximum affinity maturation during HIV Env immunization

doi: 10.1016/j.isci.2024.109495

Figure Lengend Snippet:

Article Snippet: Biotinylated Anti-mouse IgD , Southern Biotech , Cat #1120-08 RRID: AB_2631189.

Techniques: Blocking Assay, Recombinant, Staining, Transfection, Sequencing, Clinical Proteomics, Plasmid Preparation, Software, Magnetic Beads

Journal: Nature Communications

Article Title: The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21

doi: 10.1038/s41467-021-27477-0

Figure Lengend Snippet: A – C WT and Il21r −/− mice were immunized i.p. with NP-KLH in alum and on day 7, spleen NP-binding GC B cells (CD138 − IgM − IgD − Gr1 − B220 + NP + IgG1 + CD38 − ) were sorted and RNA-seq performed. A Mean-difference plot of WT vs Il21r −/− GC B cells depicts genes that were significantly upregulated in WT compared to Il21r −/− GC B cells shown in red and significantly downregulated ones in blue (false discovery rate <0.05). B Gene expression fold changes in WT compared to Il21r −/− GC B cells. Significantly DE genes were ranked by FDR and the 50 lowest then ordered by FC, colored red if upregulated in WT compared to Il21r −/− , blue if downregulated. C , D limma barcode plots testing for GC zone signature or cMyc signature genes among DE genes from WT vs Il21r −/− GC B cells as in A . Genes were ranked left to right as most up in Il21r −/− to most up in WT GC. For zone signature ( C ), vertical red bars mark DZ signature genes while vertical blue bars mark LZ signature genes. Worms show relative enrichment of DZ and LZ signature genes and camera p values assess significance. The plot shows DZ genes enriched among those up in WT while LZ genes were enriched among those up in Il21r −/− GC. For the Myc signature ( D ), vertical red bars mark upregulated genes in Myc + cells while vertical blue bars mark genes upregulated in Myc − cells. Worms show relative enrichment of Myc signature genes and camera p values assess significance. The plot shows Myc +- associated genes were significantly up in WT GC B cells.

Article Snippet: GC B cell was enriched by magnetic sorting by staining with biotinylated antibodies to GR1 (clone 8C5, WEHI Antibody Facility; 1 in 200 dilution), IgD (clone 11-26, Southern Biotech, Cat. 1120-08; 1 in 200 dilution), CD4 (clone GK1.5, WEHI Antibody Facility; 1 in 200 dilution), CD8 (clone YTS.169, WEHI Antibody Facility; 1 in 200 dilution), and CD138 (clone 281-2, BD Biosciences, Cat. 553713; 1 in 200 dilution) followed by staining with anti-biotin microbeads (Miltenyi Biotec, Cat. 130-090-485; 1 in 200 dilution) and depletion using LS Columns (Miltenyi Biotec, Cat. 130-042-401) supported by a MACS separator magnetic stand (Miltenyi Biotec).

Techniques: Binding Assay, RNA Sequencing, Gene Expression

Journal: Cell Reports Medicine

Article Title: An Agonistic Anti-CD137 Antibody Disrupts Lymphoid Follicle Structure and T-Cell-Dependent Antibody Responses

doi: 10.1016/j.xcrm.2020.100035

Figure Lengend Snippet:

Article Snippet: Biotinylated rat anti-mouse IgD mAb , SouthernBiotech , Cat#1120-08; RRID: AB_2631189.

Techniques: Purification, Virus, Recombinant, Plasmid Preparation, Adjuvant, Cell Isolation, Multiplex Assay, RNA Sequencing, Gene Expression, Software

Journal: PLoS ONE

Article Title: The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process

doi: 10.1371/journal.pone.0220933

Figure Lengend Snippet: Single cell suspensions of spleen from rats were stained with FITC conjugated anti-rat IgM, biotinylated anti-rat IgD, APC conjugated anti-rat CD90/Thy1.1 and PE-conjugated anti-rat TCRαβ, TCRγδ, CD161a/NKRa. Biotinylated monoclonal antibodies were revealed with streptavidin conjugated to the tandem fluorochrome PE-Cy5.5. Viable lymphocytes were gated by forward scatter and side scatter profiles. Acquisition gates were set to exclude PE positive cells (T cells and NK cells) and CD90 positive (immature) B cells. Mature FO-B cells, defined as CD90 - IgD high IgM low and MZ-B defined as CD90 - IgM high IgD low were sorted. Post sort reanalysis showed that the purity of FO-B cells and MZ-B cells was >95%.

Article Snippet: Briefly, spleen cell suspensions from 2 adult male rats and from 5 pooled spleens of two day old neonatal rats were separately prepared and labeled for flow-cytometry with the following mouse monoclonal antibodies: FITC conjugated anti-rat IgM (HIS40; eBioscience, San Diego, CA, USA), biotinylated anti-rat IgD (MaRD3; AbD Serotec, Oxford, UK), APC conjugated anti-rat CD90/Thy1.1 (HIS51; eBioscience) and PE-conjugated anti-rat TCRαβ (R73; eBioscience); TCRγδ (V65; eBioscience), CD161a/NKRP1a (10/78; BD Pharmingen).

Techniques: Staining

Journal: PLoS ONE

Article Title: The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process

doi: 10.1371/journal.pone.0220933

Figure Lengend Snippet: A single neonatal rat splenic cell suspension was stained with FITC conjugated anti-rat IgM (HIS40; eBioscience, San Diego, CA, USA), biotinylated anti-rat IgD (MaRD3; AbD Serotec, Oxford, UK), and APC anti-rat CD90/Thy1.1 (HIS51; eBioscience). Biotinylated mAb were revealed with streptavidin conjugated to the tandem fluorochrome PE-Cy5.5 (Ebioscience). Lymphocytes were sequentially gated by forward scatter and side scatter. Acquisition gates were set to exclude the unwanted immature B cells (CD90 + -APC). Gate settings were set appropriately for FO-B cells (CD90 neg IgD high IgM low ) and MZ-B (CD90 neg IgM high IgD low ) cells.

Article Snippet: Briefly, spleen cell suspensions from 2 adult male rats and from 5 pooled spleens of two day old neonatal rats were separately prepared and labeled for flow-cytometry with the following mouse monoclonal antibodies: FITC conjugated anti-rat IgM (HIS40; eBioscience, San Diego, CA, USA), biotinylated anti-rat IgD (MaRD3; AbD Serotec, Oxford, UK), APC conjugated anti-rat CD90/Thy1.1 (HIS51; eBioscience) and PE-conjugated anti-rat TCRαβ (R73; eBioscience); TCRγδ (V65; eBioscience), CD161a/NKRP1a (10/78; BD Pharmingen).

Techniques: Staining